Fig 1: Concordant and discordant regulation of known target genes by isomiR-31s.The sequences of isomiRs of miR-31. MiR-31-H, miR-31-P, and miR-31-M represent hsa-miR-31, ptr-miR-31, and mmu-miR-31 in miRBase, respectively (A). CEBPa, STK40, and E2F2 mRNA expression in MDA-MB-231 cells (B), MCF-7 cells (C), and in HCT116 cells (D) were detected by RT-qPCR after transfection with synthetic oligos of isomiR-31s. The mRNA level of each gene was normalized to GAPDH mRNA. The normalized mRNA level of Neg-ctrl transfectant was set as 1.0 and then those of other isomiR-31 transfections were relative to it. The proteins levels of Fzd3 (E), MMP16 (F), and MCM2 (G) were determined in MDA-MB-231 cells transfected with 100 nM synthetic oligos by immunoblotting. GAPDH protein served as the internal control for normalization. The normalized protein level of Neg-ctrl transfectant was set as 1.0 for comparison to those of isomiR-31 transfectants. The data represent the average of 3 independent experiments with standard deviations (*P<0.05; **P<0.01; ***P<0.001, t-test).